ABSTRACT
Objective To observe the effects of Zuogui Jiangtang Jieyu Formula (ZJJF) on the ability of learning and memory and the expressions of JNK, Bcl-2 and Bax in hippocampus in diabetic rats with depression; To explore the protective mechanism of hippocampal damage in diabetic rats with depression. Methods High-fat gavage combined with intravenous injection of STZ was used to establish the model of diabetic rats. 28 days of chronic stress was given continuously and diabetic rats complicated with depression were built successfully. Then rats were randomly divided into 6 groups, including normal, model, positive medicine, high-, medium-, and low-dose of ZJJF groups. After the last administration, Morris water maze was used to detect escape latency time;Western blot was used to disclose the protein expressions of JNK, Bcl-2 and Bax in rat hippocampaus;RT-PCR was used to test the gene expressions of JNK and Bcl-2 and Bax. Results Compared with the normal group, escape latency time in model rats was significant longer (P<0.01), the protein and gene expression of JNK and Bax in rat hippocampaus significantly increased, Bcl-2 was markedly decreased (P<0.05, P<0.01);Compared with the model group, escape latency time in positive medicine group and high-dose of ZJJF group was significant shorter (P<0.01), the protein and gene expressions of JNK and Bax significantly decreased, and Bcl-2 markedly increased (P<0.05, P<0.01). Conclusion ZJJF can significantly improve the ability of learning and memory in diabetic rats with depression, which might be associated with preventing neuronal apoptosis in hippocampus.
ABSTRACT
OBJECTIVE:To synthesize ternary complex of rare earth with L-Leucine and imidazoles and to determine its properties.METHODS:The ternary solid complex was synthesized from the reaction of rare earth chlorate with L-Leucine and imidazoles in the medium of alcohol,and the compositional analysis,properties,mechanism of thermolysis,thermostability and the bacteriostatic action of the ternary complex were studied by chemistry analysis,chemical elements analysis,infrared spectral(IR)analysis,molar conductivity measurement and derivative thermogravimetry(TG-DTG)analysis,bioactivity experiments etc.RESULTS:The constitute structure of the ternary complexes was detected to be electrolyte1∶3type RE(Leu) 3 Im(H 2 O)Cl 3 ?2H 2 O,meanwhile,the thermolysis dynamics,thermostability and the bacteriostatic action against E coli of the ternary complex were established preliminarily.CONCLUSION:The composition and the property of the ternary complexes are stable and which were found to be of good bacteriostatic action in the preliminary examination.